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KMID : 0903520050480040339
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 4 p.339 ~ p.344
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Isolation of Mutants Susceptible to Rice Blast from DEB-treated Rice Population
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Kim Hye-Kyung
Lee Sang-Kyu
Han Mu-Ho
Jeon Yong-Hee
Lee Gi-Hwan
Lee Youn-Hyung
Bhoo Seong-Hee
Hahn Tae-Ryong
Jeon Jong-Seong
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Abstract
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Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify genes involving in the signal transduction pathways that mediate rice blast resistance, we screened over 2,000 mutant lines of a highly resistant variety RIL260 that were generated by using a DEB (1, 3-Butadiene diepoxide) treatment method. In the mutant population, the frequency of albino plants was 6.7%, indicating that this population has a high frequency of mutations in the genome. The primary screening identified 29 mutant plants that exhibit a complete or partial loss of the resistance to rice blast. Among them, M5465, the most susceptible line, was subsequently examined by DNA gel-blot experiments using DNA molecular markers of Pi5(t) that has been previously identified as a durable resistance locus in RIL260. The result revealed that a large deletion and rearrangement of genomic DNA occurred in the Pi5(t) locus. The results suggest that DEB can be used as an efficient mutagen to induce large scale mutations in the rice genome. The isolated mutants should be useful for elucidating the Pi5(t)-mediated signaling pathways of rice blast resistance.
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KEYWORD
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Oryza sativa, Magnaporthe grisea, DEB, mutant, Pi5(t)
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